DNA

Part:BBa_K5086018

Designed by: Zhaohui Zhang   Group: iGEM24_BUCT-China   (2024-09-24)


Universalness_crRNA

Universalness_crRNA (BBa_K5086018) is a single-stranded DNA sequence with 42 nucleotides. universalness_crRNA can activate the paracrine activity of cas12A enzyme in conjunction with polymerase-mediated strand substitution of the exiting wt strand to perform a non-specific cleavage that cuts the reporter probe strand into two segments, allowing for the restoration of fluorescence that release of the fluorescent signal.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None